Petri Dish Cleaning Procedure

发布时间：2021-11-12

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　　Typically, the process involves four steps: soaking, scrubbing, acid immersion, and rinsing.

　　1. Soaking: New or previously used glassware should first be soaked in clean water to soften and dissolve any adhering residues. Before use, new glassware should be briefly rinsed with tap water, then soaked overnight in 5% hydrochloric acid. Used glassware often contains substantial amounts of protein and grease; once dried, these substances are difficult to remove by scrubbing, so such items should be immediately immersed in clean water after use for subsequent cleaning.

　　2. Scrubbing: Place the soaked glassware in a detergent solution and scrub thoroughly with a soft-bristled brush, ensuring no死角 are left and avoiding damage to the surface finish. Rinse the cleaned glassware thoroughly, allow it to air-dry, and set it aside for acid soaking.

　　3. Acid Leaching: Acid leaching involves immersing the aforementioned glassware in a cleaning solution—also known as an acid bath—to remove any residual substances on the surface through the strong oxidizing action of the acid. The leaching process should last at least six hours, typically overnight or longer. Handle the glassware with care when removing it from the solution.

　　4. Rinsing: All glassware, whether washed by brushing or acid‑soaked, must be thoroughly rinsed with water. The thoroughness of rinsing after acid treatment directly affects the success or failure of cell culture. For manually washing acid‑treated glassware, each piece should be repeatedly “filled with water and drained” at least 15 times; finally, rinse 2–3 times with doubly distilled water, then allow to air‑dry or dry in an oven before packaging for storage.